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Advancing Environmental Surveillance through RT-PCR Based Molecular Monitoring

Discover reliable RT-PCR kits for water, wastewater, and environmental antimicrobial resistance surveillance.
INQUIRE MORE
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Manufacturing Excellence in Environmental
Surveillance RT-PCR Kits

At Huwel Lifesciences, we apply our expertise in DNA and RNA-based assay systems to support environmental surveillance and antimicrobial resistance monitoring. Our Quantiplus® Environmental Surveillance RT-PCR kits are designed for the detection and semi-quantitative analysis of antimicrobial resistance genes and key microbial targets from different water sources.
These kits support laboratories, public health programs, municipalities, research institutions, and environmental monitoring teams with reliable molecular tools for surveillance, risk assessment, and informed action.

Key Highlights

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Broad Environmental Coverage

Designed for different water sources — drinking water, healthcare facility effluents, sewage, food crop irrigation water, and water from recreational sources.
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AMR Gene Detection

Supports detection of important antimicrobial resistance markers: carbapenemases, ESBL genes, MRSA markers, vancomycin resistance genes, colistin resistance genes, and other resistance-associated targets.
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Semi-Quantitative RT-PCR

Enables real-time PCR based detection and semi-quantitative analysis to support surveillance, trend monitoring, and risk assessment.
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Workflow-Ready Solution

Validated with Huwel’s DNA Extraction Kit for Waste Water, supporting a structured workflow from sample processing to amplification and result interpretation.

Comprehensive Instrumentation

RT-PCR Instruments

Huwel environmental surveillance kits are validated on widely used real-time PCR systems, including Thermo QS5 and Bio-Rad CFX96.

DNA Extraction Workflow

The DNA Extraction Kit for Waste Water supports isolation of high-purity DNA from environmental water samples for PCR and RT-PCR applications.

In-House Assay Expertise

Our proficiency in molecular assay development enables us to design and manufacture reliable RT-PCR kits for complex environmental surveillance applications.

Quality-Controlled Manufacturing

Our manufacturing processes are supported by a robust quality management system to ensure consistent kit performance and batch-to-batch reliability.
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Environmental Surveillance Applications

Huwel Environmental Surveillance RT-PCR kits are designed for a wide range of monitoring and research applications.
antimicrobial

Antimicrobial Resistance Surveillance

Monitor AMR genes in wastewater, healthcare effluents, and environmental water sources.
pathogen

Municipal Wastewater Monitoring

Support city-level and regional surveillance programs for environmental AMR trends.
durability

Healthcare Facility Effluent Monitoring

Track resistance markers and microbial targets in hospital and healthcare wastewater streams.
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Industrial & Pharmaceutical Effluent

Support environmental compliance and risk monitoring for biotech, pharmaceutical, and industrial facilities.
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Irrigation & Recreational Water​

Assess microbial and AMR-related risks in food crop irrigation water and recreational water sources.

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Research & Public Health Programs​

Enable molecular surveillance for One Health, environmental health, and AMR containment programs.

Aquaculture Monitoring

Support water quality and microbial surveillance in aquaculture farms, hatcheries, ponds, and aquatic production environments.

Food & Agriculture Monitoring

Enable molecular monitoring of water, soil, irrigation sources, and agricultural environments to support food safety and One Health surveillance.

Environmental Surveillance Workflow

1
Sample
Collection Collect wastewater or environmental water samples using the Waste Water Sample Collection Kit.
Catalogue: ENV-SCK
2
Sample
Pre-Processing Prepare and concentrate environmental samples using the Waste Water Sample Processing Kit. Catalogue: ENV-SNAC
3
DNA
Extraction Isolate high-purity DNA using the DNA Extraction Kit for Waste Water.
Cat No.: ENV-NAX-50/55
Pack: 50/55 Extractions
4
Real-Time
PCR Run the selected Quantiplus®️ Environmental Surveillance RT-PCR kit on a validated real-time PCR instrument.
Kit options:
Cat No.: QL-AMES-25/50/100
Cat No.: ENV-AMR-25/50/100
5
Result
Interpretation Interpret Ct values and target-specific amplification signals to support environmental surveillance and semi-quantitative AMR analysis.

Diverse Environmental Surveillance Solution

Huwel Lifesciences has developed environmental surveillance kits designed for antimicrobial resistance monitoring and molecular surveillance of water samples.
antimicrobial

Antimicrobial Resistance Monitoring

Detect resistance genes — carbapenemases, ESBLs, MRSA, vancomycin resistance, colistin resistance, and other AMR mechanisms.
pathogen

Pathogen & Marker Detection

Monitor selected microbial targets and resistance-associated markers from environmental water sources.
gene detection

Wastewater DNA Extraction

Support efficient isolation of DNA from wastewater and other water samples for molecular biology applications.

Explore Tailored Kits Designed for
Environmental Surveillance Applications

Quantiplus® AMR Kit for Environmental Surveillance

Cat No.: QL-AMES-25/50/100

Pack: 25/50/100 Rxns

Quick Reference
Quantiplus® Major AMR Kit for Environmental Surveillance

Cat No.: ENV-AMR-25/50/100

Pack: 25/50/100 Rxns

Quick Reference
DNA Extraction Kit for Waste Water

Cat No.: ENV-NAX-50/55

Cat No.: 50/55 Extractions

Quick Reference

Product Overview

Quantiplus® AMR Kit for Environmental Surveillance

The Quantiplus® AMR Kit for Environmental Surveillance is a real-time semi-quantitative PCR kit designed for detection and semi-quantitative analysis of antimicrobial resistance related genes from various water sources.
It supports detection of key AMR markers, including carbapenemase genes, MRSA markers, ESBL genes, vancomycin resistance genes, MTB, and mobilized colistin resistance genes.
Key Targets Include:
NDM, IMP, OXA, KPC, VIM, mecA, nuc, pvl, SHV, CTX-M, CMY, Van A, Van B, Van M, MTB, and mcr1 to mcr10.
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Quantiplus® Major AMR Kit for Environmental Surveillance

The Quantiplus® Major AMR Kit for Environmental Surveillance is an expanded real-time semi-quantitative PCR kit for comprehensive AMR and microbial marker surveillance from environmental water samples.
It supports a broader target panel for monitoring antimicrobial resistance genes, selected pathogens, and resistance-associated markers relevant to environmental and public health surveillance.
Key Targets Include:
Carbapenemase genes, ESBL genes, MRSA markers, vancomycin resistance genes, colistin resistance genes, MTB, Klebsiella pneumoniae, Streptococcus pneumoniae, Pseudomonas aeruginosa, Salmonella typhi, Salmonella paratyphi, Acinetobacter baumannii, Enterococcus faecium, chloramphenicol resistance, trimethoprim resistance, tetracycline resistance, sulfonamide resistance, typical enteropathogenic E. coli, and gyrase mutation markers.
major amr kit

DNA Extraction Kit for Waste Water

The DNA Extraction Kit for Waste Water is designed for molecular biology applications such as PCR and RT-PCR. It provides a fast, economical, and easy method for isolation of high-purity DNA from environmental water samples.
It can be used for samples from drinking water, healthcare facility effluents, sewage, food crop irrigation water, and recreational water sources.
dna extraction kit for waste water

Technical Specifications

Assay TypeReal-Time Semi-quantitative PCR
ApplicationEnvironmental surveillance and AMR monitoring
Sample SourcesDrinking water, healthcare effluents, sewage, irrigation water, recreational water
Target TypeAMR genes, microbial markers, selected pathogen targets
WorkflowSample collection → pre-processing → DNA extraction → RT-PCR → result interpretation
Extraction KitDNA Extraction Kit for Waste Water
Validated
Instruments		Thermo QS5 Real-Time PCR System, Bio-Rad CFX96
ControlsPositive controls, mol. biology grade water, internal controls
RT-PCR Pack Sizes25 / 50 / 100 reactions
Extraction Pack Sizes50 / 55 extractions

Why Huwel Environmental Surveillance Kits?

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Reliable Molecular Surveillance

Designed to support detection of important AMR genes and environmental microbial markers.
Serves-a-wide-range-of- tests​

Broad Water Source Compatibility

Tailored oligos with modifications such as phosphorylation, labeling, or conjugation for specialized experiments.

Validated
Workflow

Supports an end-to-end workflow using Huwel’s DNA extraction and RT-PCR kit system.
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Scalable for Public Health Programs

Suitable for laboratories, municipalities, public health networks, research institutions, and environmental monitoring programs.
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Made in India

Manufactured by Huwel Lifesciences with strong in-house molecular diagnostics expertise and quality-controlled production.
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Excellence Recognized

Huwel Lifesciences has built strong capabilities in RT-PCR kit development, manufacturing, and molecular diagnostics. Our in-house expertise across assay design, oligonucleotide synthesis, enzymes, reagents, and kit manufacturing enables us to support reliable and scalable molecular testing solutions.
With a strong focus on quality, accessibility, and manufacturing excellence, Huwel continues to support diagnostic, research, and public health needs across India and international markets.

Explore Our Comprehensive Range of Environmental Surveillance RT-PCR Kits

For inquiries, more information, or detailed discussions about our Environmental Surveillance RT-PCR kits, please feel free to contact our dedicated team. We’re here to help.
Contact Us